| Gene name |
SPCC285.10c |
| Gene ID |
18/B08 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; hypothetical protein; SPRY domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1149 |
| ORF length (spliced) |
|
| Entry clone length |
1149 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC285.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCATCCCGATCAGATTCG |
| Rev primer name |
SPCC285.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACTTCATCGGTAGCAGGC |
| Amino acid length |
382 |
| Molecular weight |
42.8 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAAVIVLLI |
| Localization (YFP) |
cytoplasmic dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|