| Gene name |
SPAC22E12.07 |
| Gene ID |
18/C11 |
| Gene synonyms/obsolete |
rna1 |
| Gene product |
Ran GTPase activating
protein (RanGAP); leucine-rich repeat protein; functionally
complements Sc RNA; involved in mRNA processing |
| Entry clone |
Cloned (also cloned in
2004 trial) |
| ORF length (unspliced) |
1161 |
| ORF length (spliced) |
|
| Entry clone length |
1161 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC22E12.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGCGTTTTTCAATAGA |
| Rev primer name |
SPAC22E12.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATATGAGCTTTTGATAGC |
| Amino acid length |
386 |
| Molecular weight |
43.2 |
| Isoelectric point (calc.) |
4.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
nucleus>cytosol, partially |
| Microscope used for
observation |
DeltaVision |