Gene name |
SPBC3B9.11c |
Gene ID |
18/E09 |
Gene synonyms/obsolete |
ctf1 |
Gene product |
involved in
transcription termination; involved in polyadenylation; rrm
RNA recognition motif; RNA-binding protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1171 |
ORF length (spliced) |
1092 |
Entry clone length |
1171 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC3B9.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCGATGACAGCCGGGAA |
Rev primer name |
SPBC3B9.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTGTCGAAAATGCTGCCGA |
Amino acid length |
363 |
Molecular weight |
39.9 |
Isoelectric point (calc.) |
8.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|