| Gene name |
SPAC16.01 |
| Gene ID |
18/F05 |
| Gene synonyms/obsolete |
rho2 |
| Gene product |
Rho family; small
GTPase; involved in cellular morphogenesis; involved in
septation; non-essential; overexpression results in loss of
cell polarity and is lethal |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1175 |
| ORF length (spliced) |
603 |
| Entry clone length |
1175 |
| No. of intron |
8 |
| Sequence status |
Finished |
| Sequence results |
173T:G / 215G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC16.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTTGCAATCTCAACCGAT |
| Rev primer name |
SPAC16.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGAAATGATGCAGCATTTT |
| Amino acid length |
200 |
| Molecular weight |
22.2 |
| Isoelectric point (calc.) |
4.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|