Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22E12.01
Gene ID 18/F06
Gene synonyms/obsolete SPAC890.09
Gene product triose phosphate transporter
Entry clone Cloned
ORF length (unspliced) 1176
ORF length (spliced) 1125
Entry clone length 1176
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC22E12.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGACAGATCCGATTTT
Rev primer name SPAC22E12.01.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCATTTTCATTCAAAACA
Amino acid length 374
Molecular weight 42.3
Isoelectric point (calc.) 5
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 10
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LAWYFFSLLL/LRWALTQKLLL/LTPLMFLFL
Localization (YFP) Golgi
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.