Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC23G7.10c
Gene ID 18/H10
Gene synonyms/obsolete
Gene product NADH-dependent flavin oxidoreductase
Entry clone Cloned
ORF length (unspliced) 1188
ORF length (spliced)
Entry clone length 1188
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC23G7.10.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTATTGTTAATGAAGG
Rev primer name SPBC23G7.10.Rv
Rev primer SEQ AGAAAGCTGGGTAACGTAACTTTCTGTGTCCC
Amino acid length 395
Molecular weight 43.6
Isoelectric point (calc.) 6.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Confocal

Image information
YFP 1 images) See all images

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