Gene name |
SPCC297.01 |
Gene ID |
18/H11 |
Gene synonyms/obsolete |
SPCC1223.14 |
Gene product |
chorismate synthase;
involved in aromatic amino acid biosynthesis |
Entry clone |
Cloned |
ORF length (unspliced) |
1188 |
ORF length (spliced) |
|
Entry clone length |
1188 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1101A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC297.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTCCTTCGGCACTTT |
Rev primer name |
SPCC297.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTGAGCATTGGGGAGTAGG |
Amino acid length |
395 |
Molecular weight |
42.3 |
Isoelectric point (calc.) |
6.9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGTPIGMLVL |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal
|