| Gene name |
SPAC607.09c |
| Gene ID |
18/H12 |
| Gene synonyms/obsolete |
|
| Gene product |
battenin family
protein; involved in cellular pH homeostasis; involved in
vacuolar pH control; similar to human CLN3 (mutated in
Batten's disease) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1191 |
| ORF length (spliced) |
|
| Entry clone length |
1191 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
36A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC607.09.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTAAATTGAGGTTAAC |
| Rev primer name |
SPAC607.09.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGTTAAGGCACACCAATCT |
| Amino acid length |
396 |
| Molecular weight |
44 |
| Isoelectric point (calc.) |
8.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
8 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFTILQSALYL/LASLVSLFL |
| Localization (YFP) |
ER |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|