Gene name |
SPBC902.06 |
Gene ID |
19/A09 |
Gene synonyms/obsolete |
|
Gene product |
MT organizer;
coiled-coil region; non-essential; no apparent orthologs; Mto2
and the EMTOC are critical for anchoring the cytokinetic actin
ring to the medial region of the cell, and for the proper
coordination of mitosis with cytokinesis; Mto2 promotes the
ability of Mto1 to recruit the gamma tubunlin complex to a
subset of MTOCs away from the spindle pole body |
Entry clone |
Cloned |
ORF length (unspliced) |
1194 |
ORF length (spliced) |
|
Entry clone length |
1194 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC902.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTGAACATAATTACCA |
Rev primer name |
SPBC902.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGGGGAAGGAGTGTCTTGA |
Amino acid length |
397 |
Molecular weight |
44 |
Isoelectric point (calc.) |
6.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCRSLAELCL |
Localization (YFP) |
SPB?; periphery at
site of septum formation; cytosol; cytoplasmic dots by over
expression |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |