| Gene name |
SPAC1002.18 |
| Gene ID |
19/B08 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; fungal conserved protein (Gaillardin et al);
similar to N. crassa 3H10.120; no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1200 |
| ORF length (spliced) |
|
| Entry clone length |
1200 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
228T:C / 1167A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC1002.18.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTACAGAGACCGTTGC |
| Rev primer name |
SPAC1002.18.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAACAAGGTACCATCACTT |
| Amino acid length |
399 |
| Molecular weight |
44.2 |
| Isoelectric point (calc.) |
6.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LWSELQTLLI/LTQWLTYSLLI/LFVDLGVLTL/LKPELSEPLSL |
| Localization (YFP) |
cytosol=nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Confocal
|