| Gene name |
SPAC2F3.03c |
| Gene ID |
20/D11 |
| Gene synonyms/obsolete |
rpa49 |
| Gene product |
DNA-directed RNA
polymerase I (subunit) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1278 |
| ORF length (spliced) |
|
| Entry clone length |
1278 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
413T:G / 787T:C /
1076T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC2F3.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAGGTGACGAGTTAAA |
| Rev primer name |
SPAC2F3.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTTCTAGCGCGTCCACGT |
| Amino acid length |
425 |
| Molecular weight |
48 |
| Isoelectric point (calc.) |
10.1 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleolus>nucleus
|
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |