Gene name |
SPAC18B11.05 |
Gene ID |
20/E04 |
Gene synonyms/obsolete |
|
Gene product |
eukaryotic conserved
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1281 |
ORF length (spliced) |
|
Entry clone length |
1281 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
640T:C / 1090T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC18B11.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAATTAAACGAATCCGC |
Rev primer name |
SPAC18B11.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCAGGTGGTAGAAAAGAT |
Amino acid length |
426 |
Molecular weight |
48.8 |
Isoelectric point (calc.) |
9.6 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
8 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSTILYLGI/LFAAFSFLGL/LKISLERLQL/LNRMSSALPL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |