Gene name |
SPAC823.11 |
Gene ID |
20/G06 |
Gene synonyms/obsolete |
|
Gene product |
sphingoid
base-phosphate phosphatase; dihydrosphingosine-1-P; putative
sphingolipid metabolism |
Entry clone |
Cloned in 2006
trial |
ORF length (unspliced) |
1344 |
ORF length (spliced) |
1236 |
Entry clone length |
1344 |
No. of intron |
2 |
Sequence status |
Partially
sequenced |
Sequence results |
100% match in both
ends |
Comments |
|
Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
Fwd primer name |
SPAC823.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTCCATAAAAAAAAAAA |
Rev primer name |
SPAC823.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATATCTTCCACTTTAAGAAG |
Amino acid length |
411 |
Molecular weight |
46.8 |
Isoelectric point (calc.) |
9 |
Signal SEQ |
|
No. of transmembrane domain |
7 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LRFQLRELIL/LGTHVFFMLAL/LLSLVLLYI/LPPIFKSLRL |
Localization (YFP) |
no expression clone
|
Comments for localization |
|
Effect of LMB on protein
localization |
not determined |
Microscope used for
observation |
|