| Gene name |
SPAC23C4.05c |
| Gene ID |
20/H06 |
| Gene synonyms/obsolete |
|
| Gene product |
Sp specific families;
hypothetical protein; similar to Sp SPBC365.12c (C-term); no
apparent orthologs |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1350 |
| ORF length (spliced) |
1296 |
| Entry clone length |
1350 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
360A:G / 466T:C /
490C:T |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC23C4.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAGCCAAGGTTTCTTCT |
| Rev primer name |
SPAC23C4.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTGCGAAAGTACAAAAGCC |
| Amino acid length |
431 |
| Molecular weight |
49.5 |
| Isoelectric point (calc.) |
6.8 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LHGALLALGI |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |