| Gene name |
SPAC644.07 |
| Gene ID |
20/H07 |
| Gene synonyms/obsolete |
|
| Gene product |
AAA family ATPase;
involved in the expression of functional Rieske iron-sulfur
protein; involved in iron metabolism; chaperone; involved in
mitochondrial respiratory chain complex III assembly; human
disease GRACILE syndrome ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1350 |
| ORF length (spliced) |
|
| Entry clone length |
1350 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
577A:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC644.07.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATAATATAGGTGCTGC |
| Rev primer name |
SPAC644.07.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATACAGACAAAGACTTTCTG |
| Amino acid length |
449 |
| Molecular weight |
49.8 |
| Isoelectric point (calc.) |
9.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LMGFGAGLAI/LDYDICVLNL |
| Localization (YFP) |
mitochondrion |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |