Gene name |
SPCC24B10.02c |
Gene ID |
20/H09 |
Gene synonyms/obsolete |
|
Gene product |
NAD/NADH kinase; ATP
dependent; involved in nicotinamide metabolism; similar to Sp
SPAC3H5.11 and SPAC1B1.02C |
Entry clone |
Cloned |
ORF length (unspliced) |
1350 |
ORF length (spliced) |
|
Entry clone length |
1350 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
129A:G / 762G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC24B10.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTACCAAAGTTGAGGA |
Rev primer name |
SPCC24B10.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACCATCAATGTCTGCAGAA |
Amino acid length |
449 |
Molecular weight |
50.6 |
Isoelectric point (calc.) |
7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nuclear dots;
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |