Gene name |
SPAC13F5.03c |
Gene ID |
20/H10 |
Gene synonyms/obsolete |
|
Gene product |
glycerol
dehydrogenase; bacterial-like; no apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1353 |
ORF length (spliced) |
|
Entry clone length |
1353 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1146A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC13F5.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATTGGTCCTCGTCTTTG |
Rev primer name |
SPAC13F5.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGGATGAATGTCGGTCAAG |
Amino acid length |
450 |
Molecular weight |
49.4 |
Isoelectric point (calc.) |
7.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPITLEELGI |
Localization (YFP) |
mitochondrion |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |