Gene name |
SPBC18A7.01 |
Gene ID |
21/A09 |
Gene synonyms/obsolete |
SPBC4F6.19c |
Gene product |
aminopeptidase;
dipeptidase; metallopeptidase; peptidase family M24; no
apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1356 |
ORF length (spliced) |
|
Entry clone length |
1356 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1241A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC18A7.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTTCTTTTGAATCAAG |
Rev primer name |
SPBC18A7.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGGCTCGTAGGGACTTTTT |
Amino acid length |
451 |
Molecular weight |
50.5 |
Isoelectric point (calc.) |
5.7 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLALSFLSL/LYDRLGPLKL |
Localization (YFP) |
ER; Golgi |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |