| Gene name |
SPCC622.12c |
| Gene ID |
21/A10 |
| Gene synonyms/obsolete |
|
| Gene product |
NADP-specific
glutamate dehydrogenase; involved in glutamate biosynthesis,
using glutamate dehydrogenase (NAD(P)+); involved in nitrogen
metabolism; glutamate dehydrogenase (NADP+) activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1356 |
| ORF length (spliced) |
|
| Entry clone length |
1356 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC622.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTACCCCATACGAACC |
| Rev primer name |
SPCC622.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCACCAGTCACCAACGGCT |
| Amino acid length |
451 |
| Molecular weight |
48.7 |
| Isoelectric point (calc.) |
7.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol |
| Comments for localization |
around spore? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |