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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC24B10.15
Gene ID 21/E03
Gene synonyms/obsolete
Gene product hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1389
ORF length (spliced)
Entry clone length 1389
No. of intron 0
Sequence status Finished
Sequence results 279T:C / 1258T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC24B10.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAAATAGACGACCAGGC
Rev primer name SPCC24B10.15.Rv
Rev primer SEQ AGAAAGCTGGGTAAGATAAAATACGTTCTCGG
Amino acid length 462
Molecular weight 52.3
Isoelectric point (calc.) 6.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization occasionally cytoplasmic dots
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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