Gene name |
SPAC3A12.01c |
Gene ID |
21/E11 |
Gene synonyms/obsolete |
SPAC20G8.10c |
Gene product |
involved in autophagy;
beclin related |
Entry clone |
Cloned |
ORF length (unspliced) |
1395 |
ORF length (spliced) |
|
Entry clone length |
1395 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
730T:C / 1128A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC3A12.01.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAGTATTTGTGTCAGAG |
Rev primer name |
SPAC3A12.01.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAAAGTTCGACTGCTTGTCC |
Amino acid length |
464 |
Molecular weight |
53.9 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytoplasmic dots |
Comments for localization |
various size of moving
dots |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |