Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1753.02c
Gene ID 21/F11
Gene synonyms/obsolete git3
Gene product glucose-triggered adenylate cyclase activation protein; glucose receptor
Entry clone Cloned#
ORF length (unspliced) 1401
ORF length (spliced)
Entry clone length 1401
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPCC1753.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTTCATTTAGATTATAC
Rev primer name SPCC1753.02.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTTCCTCACCAAATTTA
Amino acid length 466
Molecular weight 53.8
Isoelectric point (calc.) 9.8
Signal SEQ
No. of transmembrane domain 7
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSFVIPLTI/LNWSFDYALSI
Localization (YFP) periphery
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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