| Gene name |
SPAC4A8.04 |
| Gene ID |
21/F12 |
| Gene synonyms/obsolete |
isp6; prb1 |
| Gene product |
serine protease;
involved in sexual differentiation; involved in RNA
degradation via regulation of the RNA degrading activity of
Pnu1p; similar to Sp SPAC1006.01 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1404 |
| ORF length (spliced) |
|
| Entry clone length |
1404 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
910T:C / 1284T:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC4A8.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGAATTCCTTATTCAAA |
| Rev primer name |
SPAC4A8.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATTCTTGAGCACCATTGAAA |
| Amino acid length |
467 |
| Molecular weight |
49.3 |
| Isoelectric point (calc.) |
5.1 |
| Signal SEQ |
Predicted
(N-terminus) |
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
Golgi? |
| Comments for localization |
nearly no apparent
signal |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |