Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC17H9.13c
Gene ID 21/G06
Gene synonyms/obsolete
Gene product glutamate 5-kinase; PUA domain
Entry clone Cloned
ORF length (unspliced) 1409
ORF length (spliced) 1209
Entry clone length 1409
No. of intron 2
Sequence status Finished
Sequence results 765A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC17H9.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTCCGAAGTCGAAAAG
Rev primer name SPAC17H9.13.Rv
Rev primer SEQ AGAAAGCTGGGTAATGCTCATCAGAACTAACT
Amino acid length 402
Molecular weight 43.9
Isoelectric point (calc.) 8.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no apparent signal
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation DeltaVision

Image information
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