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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC61.05
Gene ID 21/G07
Gene synonyms/obsolete
Gene product Sp specific families; similar to Sp SPCC569.06
Entry clone Cloned
ORF length (unspliced) 1410
ORF length (spliced)
Entry clone length 1410
No. of intron 0
Sequence status Finished
Sequence results 84G:A / 790T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC61.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCGAATAAACTTTGTTTT
Rev primer name SPCC61.05.Rv
Rev primer SEQ AGAAAGCTGGGTACACTTTACGATCGGGCGCT
Amino acid length 469
Molecular weight 53.6
Isoelectric point (calc.) 4.7
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 7
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLVLCFGLGI/LESMESLNL
Localization (YFP) cytoplasmic dots
Comments for localization moving dots
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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