Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1753.06c
Gene ID 21/G09
Gene synonyms/obsolete SPCC162.12
Gene product hypothetical protein; serine-rich protein; sequence orphan
Entry clone Cloned
ORF length (unspliced) 1415
ORF length (spliced) 1356
Entry clone length 1415
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1753.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAACGTCCATCCCTGTC
Rev primer name SPCC1753.06.Rv
Rev primer SEQ AGAAAGCTGGGTACCCTTGAGACCATAATTGT
Amino acid length 451
Molecular weight 49.2
Isoelectric point (calc.) 10.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) periphery at site of septum formation; occasionally cytoplasmic microtubules; cytosol
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleus>cytosol)
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images
LMB 2 images) See all images

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