Gene name |
SPAC13F5.02c |
Gene ID |
22/B07 |
Gene synonyms/obsolete |
ptr6 |
Gene product |
transcription
initiation factor TFIID subunit; involved in mRNA export
(required) |
Entry clone |
Cloned |
ORF length (unspliced) |
1223 |
ORF length (spliced) |
1182 |
Entry clone length |
1223 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC13F5.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTTAAGCTAAAAATTCG |
Rev primer name |
SPAC13F5.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCAGGGTTATCGACAAGT |
Amino acid length |
393 |
Molecular weight |
45 |
Isoelectric point (calc.) |
4.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |