Gene name |
SPBC1778.03c |
Gene ID |
22/G10 |
Gene synonyms/obsolete |
|
Gene product |
NADH pyrophosphatase;
Nudix family hydrolase; involved in NADH metabolism |
Entry clone |
Cloned |
ORF length (unspliced) |
1311 |
ORF length (spliced) |
1131 |
Entry clone length |
1311 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
Mixture of 2 clones,
one of which is frameshifted from somewhere. |
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1778.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCAGGGTTTCAAAATAGC |
Rev primer name |
SPBC1778.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACATTTTAACTTGTGAATTT |
Amino acid length |
376 |
Molecular weight |
42.8 |
Isoelectric point (calc.) |
6.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |