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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22F8.09
Gene ID 22/H08
Gene synonyms/obsolete
Gene product conserved protein; similar to human GLTSCR2 glioma tumor suppressor candidate region protein2
Entry clone Cloned in 2006 trial
ORF length (unspliced) 1315
ORF length (spliced) 1260
Entry clone length 1315
No. of intron 1
Sequence status Partially sequenced
Sequence results 100% match in both ends
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC22F8.09.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGAATCAAAGAGAGGAA
Rev primer name SPAC22F8.09.Rv
Rev primer SEQ AGAAAGCTGGGTATATACTGTTTTTTTGAAGA
Amino acid length 419
Molecular weight 48.1
Isoelectric point (calc.) 10.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 213/69
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) no expression clone
Comments for localization
Effect of LMB on protein localization not determined
Microscope used for observation

Image information
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