Gene name |
SPBC106.11c |
Gene ID |
22/H09 |
Gene synonyms/obsolete |
|
Gene product |
phospholipase A2;
1-alkyl-2-acetylglycerophosphocholine esterase; involved in
glycerolipid metabolism; involved in lipid catabolism; no
apparent Sc ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1317 |
ORF length (spliced) |
|
Entry clone length |
1317 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
749A:G / 1222T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC106.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGATTGGGATTTTCTTC |
Rev primer name |
SPBC106.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACATAATTCTTTCCCACCCA |
Amino acid length |
438 |
Molecular weight |
49.8 |
Isoelectric point (calc.) |
6.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |