| Gene name |
SPBC19G7.14c |
| Gene ID |
23/A08 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein
with coiled-coil region; sequence orphan |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1325 |
| ORF length (spliced) |
1236 |
| Entry clone length |
1325 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1197T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC19G7.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGTTTCGATATGATTT |
| Rev primer name |
SPBC19G7.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGGGTTTTTTGGTGCTAAT |
| Amino acid length |
411 |
| Molecular weight |
47.5 |
| Isoelectric point (calc.) |
6.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
Golgi?; cytosol |
| Comments for localization |
moving dots |
| Effect of LMB on protein
localization |
changed to: Golgi?;
nucleus>cytosol |
| Microscope used for
observation |
DeltaVision |