Gene name |
SPCC4B3.12 |
Gene ID |
23/A10 |
Gene synonyms/obsolete |
|
Gene product |
SET domain; histone
lysine methyltransferase; chromatin associated; no apparent Sc
ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1326 |
ORF length (spliced) |
|
Entry clone length |
1326 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC4B3.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCGCCAGACGAATACTCA |
Rev primer name |
SPCC4B3.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAACTTTGATAATCAACAGCT |
Amino acid length |
441 |
Molecular weight |
51 |
Isoelectric point (calc.) |
8.4 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
SPB?; nuclear dots;
nucleus>cytosol; periphery at site of septum
formation |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |