Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC337.03
Gene ID 23/A12
Gene synonyms/obsolete
Gene product DUF618; Sc RTT103 is a regulator of Ty1 transposition
Entry clone Cloned
ORF length (unspliced) 1327
ORF length (spliced) 1164
Entry clone length 1327
No. of intron 3
Sequence status Finished
Sequence results 412C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC337.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTTTGACTCCAGACAC
Rev primer name SPBC337.03.Rv
Rev primer SEQ AGAAAGCTGGGTAGCTATCACCATATAGACCA
Amino acid length 387
Molecular weight 42.9
Isoelectric point (calc.) 4.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear dots; nucleus>>cytosol
Comments for localization 1~2 dots/nucleus
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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