Gene name |
SPAC21E11.06 |
Gene ID |
23/B01 |
Gene synonyms/obsolete |
tif224 |
Gene product |
translation initiation
factor eIF-2B delta |
Entry clone |
Cloned |
ORF length (unspliced) |
1404 |
ORF length (spliced) |
|
Entry clone length |
1404 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
75A:G / 638A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC21E11.06.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGTTCTCAGCAGAACA |
Rev primer name |
SPAC21E11.06.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGCATAAACCTGTTTGAAC |
Amino acid length |
467 |
Molecular weight |
51.5 |
Isoelectric point (calc.) |
9 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LKLEISVLDI |
Localization (YFP) |
cytosol=nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |