| Gene name |
SPAC22F3.12c |
| Gene ID |
23/C06 |
| Gene synonyms/obsolete |
rgs1 |
| Gene product |
regulator of G-protein
signalling; regulates pheromone response; involved in mating
(required) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1446 |
| ORF length (spliced) |
|
| Entry clone length |
1446 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC22F3.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCCGCCCTTCATAACCC |
| Rev primer name |
SPAC22F3.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATACCGAGCCCCCCATTC |
| Amino acid length |
481 |
| Molecular weight |
54.3 |
| Isoelectric point (calc.) |
8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LLSTLENLQL/LQLIHILLL |
| Localization (YFP) |
nucleus>>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |