Gene name |
SPAC10F6.11c |
Gene ID |
23/C08 |
Gene synonyms/obsolete |
|
Gene product |
kinase activator;
involved in autophagy; predicted coiled-coil |
Entry clone |
Cloned |
ORF length (unspliced) |
1446 |
ORF length (spliced) |
|
Entry clone length |
1446 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
959A:G / 1209T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC10F6.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTGCTACATGCGCTCACT |
Rev primer name |
SPAC10F6.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATCGCTTAGAGGCTGTAGTA |
Amino acid length |
481 |
Molecular weight |
55.4 |
Isoelectric point (calc.) |
6.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
SPB;
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |