| Gene name |
SPBC3D6.06c |
| Gene ID |
23/C10 |
| Gene synonyms/obsolete |
|
| Gene product |
ribose-phosphate
pyrophosphokinase; related to Sp SPCC1620.06c and Sc YER099C;
no apparent Sc ortholog |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1448 |
| ORF length (spliced) |
1026 |
| Entry clone length |
1448 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
225T:C / 883A:C /
1240A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC3D6.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAAAAATTTGGTTGTTTT |
| Rev primer name |
SPBC3D6.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACCCAAACTTGATTGTGG |
| Amino acid length |
341 |
| Molecular weight |
37.3 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LTESICEHLCL |
| Localization (YFP) |
bar at random
place |
| Comments for localization |
one bar/cell |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |