Gene name |
SPCC1020.03 |
Gene ID |
23/E03 |
Gene synonyms/obsolete |
|
Gene product |
CDF metal cation
transporter; involved in mitochondrial iron accumulation
|
Entry clone |
Cloned |
ORF length (unspliced) |
1456 |
ORF length (spliced) |
1194 |
Entry clone length |
1456 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
593A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC1020.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTGAAAACGTTCAATTC |
Rev primer name |
SPCC1020.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATGAGTGTGCTCATGAGAT |
Amino acid length |
397 |
Molecular weight |
43.2 |
Isoelectric point (calc.) |
9.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVTLATLKI/LTGMVSLLAL |
Localization (YFP) |
ambiguous
structure |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |