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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC19G7.13
Gene ID 23/E08
Gene synonyms/obsolete
Gene product Myb family; DNA binding protein; involved in telomere maintenance; involved in telomerase-dependent telomere maintenance
Entry clone Cloned
ORF length (unspliced) 1458
ORF length (spliced)
Entry clone length 1458
No. of intron 0
Sequence status Finished
Sequence results 1044T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC19G7.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAAAACGATCCTTAGA
Rev primer name SPBC19G7.13.Rv
Rev primer SEQ AGAAAGCTGGGTAATCTCGCCGAAGATCGCCT
Amino acid length 485
Molecular weight 54.6
Isoelectric point (calc.) 8.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LAAVYGALQI
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization changed to: intranuclear microtubule bundle (nucleus)
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images
LMB 4 images) See all images

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