Gene name |
SPBC19G7.13 |
Gene ID |
23/E08 |
Gene synonyms/obsolete |
|
Gene product |
Myb family; DNA
binding protein; involved in telomere maintenance; involved in
telomerase-dependent telomere maintenance |
Entry clone |
Cloned |
ORF length (unspliced) |
1458 |
ORF length (spliced) |
|
Entry clone length |
1458 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
1044T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC19G7.13.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAAACGATCCTTAGA |
Rev primer name |
SPBC19G7.13.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATCTCGCCGAAGATCGCCT |
Amino acid length |
485 |
Molecular weight |
54.6 |
Isoelectric point (calc.) |
8.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LAAVYGALQI |
Localization (YFP) |
nucleus |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (nucleus) |
Microscope used for
observation |
DeltaVision |