| Gene name |
SPBC14F5.10c |
| Gene ID |
23/E10 |
| Gene synonyms/obsolete |
|
| Gene product |
zinc finger protein;
zf-C3HC4 type (RING finger); ubiquitin ligase (E3); protease
LON domain; no apparent Sc ortholog |
| Entry clone |
Cloned# |
| ORF length (unspliced) |
1461 |
| ORF length (spliced) |
|
| Entry clone length |
1461 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC14F5.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGAAAAGAAAGGCCAGG |
| Rev primer name |
SPBC14F5.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCAATGGTGCAAGCACTT |
| Amino acid length |
486 |
| Molecular weight |
56.1 |
| Isoelectric point (calc.) |
6.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LSYKITNLLPI |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
cytosol=nucleus |
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |