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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC285.11
Gene ID 23/F11
Gene synonyms/obsolete
Gene product ubiquitin-regulatory protein; UBX domain
Entry clone Cloned
ORF length (unspliced) 1471
ORF length (spliced) 1284
Entry clone length 1471
No. of intron 1
Sequence status Finished
Sequence results 358A:G / 456T:C / 1184C:T / 1356T:C / 1426A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC285.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAGGGTCTCGAAAACA
Rev primer name SPCC285.11.Rv
Rev primer SEQ AGAAAGCTGGGTAATCATCCAGCTCCACCACC
Amino acid length 427
Molecular weight 48.6
Isoelectric point (calc.) 5.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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