Gene name |
SPAC5D6.07c |
Gene ID |
24/A03 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical protein;
PXA domain; involved in intracellular signaling cascade
(predicted); no ortholog |
Entry clone |
Cloned |
ORF length (unspliced) |
1488 |
ORF length (spliced) |
|
Entry clone length |
1488 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
380T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC5D6.07.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCTAAACTGTCTAGCTT |
Rev primer name |
SPAC5D6.07.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACTTAGTATGTCGTAATTCA |
Amino acid length |
495 |
Molecular weight |
56.5 |
Isoelectric point (calc.) |
9.5 |
Signal SEQ |
|
No. of transmembrane domain |
1 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LNPIISKILEI/LFYEIPFLLI |
Localization (YFP) |
cytoplasmic dots |
Comments for localization |
aggregates by over
expression |
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |