Gene name |
SPBC31E1.05 |
Gene ID |
24/B02 |
Gene synonyms/obsolete |
|
Gene product |
nuclear pore complex;
involved in RNA export |
Entry clone |
Cloned |
ORF length (unspliced) |
1493 |
ORF length (spliced) |
1443 |
Entry clone length |
1493 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC31E1.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGATACAAAAACAACACC |
Rev primer name |
SPBC31E1.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAAGGCTCAAAGGAGAATTTG |
Amino acid length |
480 |
Molecular weight |
56.2 |
Isoelectric point (calc.) |
9.5 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
119/126 |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCSIVSTYLDI |
Localization (YFP) |
nuclear envelope;
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Confocal |