| Gene name |
SPCC645.10 |
| Gene ID |
24/E08 |
| Gene synonyms/obsolete |
|
| Gene product |
tRNA
nucleotidyltransferase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1514 |
| ORF length (spliced) |
1455 |
| Entry clone length |
1514 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
592A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC645.10.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTATTCTAGAATTGTATT |
| Rev primer name |
SPCC645.10.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATATTTTTAGGGATTGAAGG |
| Amino acid length |
484 |
| Molecular weight |
54.7 |
| Isoelectric point (calc.) |
8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQSFLEDPLRI |
| Localization (YFP) |
cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
| Microscope used for
observation |
Leica |