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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1039.04
Gene ID 24/G02
Gene synonyms/obsolete
Gene product membrane transporter; unknown specificity; similar to Sp SPAC11D3.18C and SPAC1683.12 and SPAC1002.16C
Entry clone Cloned
ORF length (unspliced) 1524
ORF length (spliced)
Entry clone length 1524
No. of intron 0
Sequence status Finished
Sequence results 226A:G / 864T:C / 1094T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1039.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGAAATCTATATCATC
Rev primer name SPAC1039.04.Rv
Rev primer SEQ AGAAAGCTGGGTAGAGAAACCATCTAAAATTT
Amino acid length 507
Molecular weight 56
Isoelectric point (calc.) 8.8
Signal SEQ
No. of transmembrane domain 12
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LPLLALLYL/LCAIVIFLVL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 1 images) See all images

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