Gene name |
SPCP1E11.04c |
Gene ID |
24/G04 |
Gene synonyms/obsolete |
|
Gene product |
hypothetical
protein |
Entry clone |
Cloned |
ORF length (unspliced) |
1524 |
ORF length (spliced) |
1278 |
Entry clone length |
1524 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCP1E11.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGATGGTAATAGAGAACCC |
Rev primer name |
SPCP1E11.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTACGACTTTTTGTGGAATAAT |
Amino acid length |
425 |
Molecular weight |
46.6 |
Isoelectric point (calc.) |
10.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
periphery;
nucleolus>nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (periphery;
nucleolus>nucleus>>cytosol) |
Microscope used for
observation |
Leica |