Gene name |
SPCC417.10 |
Gene ID |
24/G09 |
Gene synonyms/obsolete |
|
Gene product |
membrane transporter;
unknown specificity; allantoate permease family; similar to Sp
SPCC757.13 and SPBC1773.15 |
Entry clone |
Cloned |
ORF length (unspliced) |
1527 |
ORF length (spliced) |
|
Entry clone length |
1527 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
86T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPCC417.10.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAAGCATCAGTATAGA |
Rev primer name |
SPCC417.10.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAAAGTGTATCTGAAATCA |
Amino acid length |
508 |
Molecular weight |
56.6 |
Isoelectric point (calc.) |
7.7 |
Signal SEQ |
|
No. of transmembrane domain |
12 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVFEFPASLLL/LSKTLCVFLVI/LIFIICGVLAI/LIAFGFLSL |
Localization (YFP) |
Golgi; periphery at
cell tip and site of septum formation; ER? |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |