| Gene name |
SPAC17A2.13c |
| Gene ID |
24/G12 |
| Gene synonyms/obsolete |
rad25; cps6 |
| Gene product |
DNA damage checkpoint
protein Rad25 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1530 |
| ORF length (spliced) |
813 |
| Entry clone length |
1530 |
| No. of intron |
1 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC17A2.13.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTAATTCTCGTGAAAA |
| Rev primer name |
SPAC17A2.13.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGCTTTAACAGTGTCAGTC |
| Amino acid length |
270 |
| Molecular weight |
30.3 |
| Isoelectric point (calc.) |
4.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
cytosol=nucleus;
periphery at cell tip and site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |