| Gene name |
SPAC12G12.14c |
| Gene ID |
24/G11 |
| Gene synonyms/obsolete |
|
| Gene product |
polyadenylation factor
I subunit; essential (pers. comm. Chris Norbury); mRNA 3'-end
processing; WD repeat protein; human ortholog fSAP57 has been
isolated with functional spliceosomes; (old-> hypothetical
Trp-Asp repeats containing protein) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1530 |
| ORF length (spliced) |
|
| Entry clone length |
1530 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC12G12.14.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAAAGAGCTGAAAATGC |
| Rev primer name |
SPAC12G12.14.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCGATAGTTTTGAGGATAC |
| Amino acid length |
509 |
| Molecular weight |
57.6 |
| Isoelectric point (calc.) |
7.4 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus>cytosol;
nuclear dots |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica,
DeltaVision |