| Gene name |
SPAC30D11.06c |
| Gene ID |
25/A08 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; eukaryotic conserved protein; DUF300 |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1544 |
| ORF length (spliced) |
1281 |
| Entry clone length |
1544 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1087T:C /
1094T:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC30D11.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGACAACGAAATAGTCGC |
| Rev primer name |
SPAC30D11.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATGTATATGAAGAAGATGCA |
| Amino acid length |
426 |
| Molecular weight |
49.2 |
| Isoelectric point (calc.) |
6.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
5 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYCFFCLLI/LVYNISITLSL |
| Localization (YFP) |
vacuole membrane |
| Comments for localization |
aggregates by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |